by using tightly regulated promoters or by attaching solubility-enhancing fusion tags. Strategies for overcoming this disadvantage of heterologous expression are the optimization of the expression system, e.g. ![]() However, the production of misfolded and inactive aggregates such as inclusion bodies is problematic when recombinant proteins are over-expressed in bacteria. coli as whole-cell biocatalyst offers additional benefits such as cofactor regeneration, high catalytic efficiency and mild and simple reaction conditions. Escherichia coli is the host of choice if post-translational modifications of the enzyme are not required for its activity. The production of catalytically active proteins in heterologous microbial and fungal hosts leads to lower costs and high yields, which is crucial for the economy of an industrial process. ![]() Traditionally, enzymes are used by food and feed industry, but are increasingly implemented in emerging markets such as pharmaceutical and fine chemicals industries. The selection of a small solubility-enhancing protein tag and exploitation of the T7 polymerase-induction system allowed the formation of increased levels of functional recombinant protein, thereby improving the performance of the whole-cell biocatalyst.Įnzymes offer tremendous opportunities for the chemical industry as they can efficiently and economically convert molecules and replace well-known and established industrial processes with more sustainable ones. The product concentration was improved 8.6-fold compared with E. coli BL21(DE3)/pET-SUMO_VvGT14ao yielded the highest titres. coli expression strains, resulting in 18 strains that were tested for glycosylation efficiency with terpenols and a phenol. ![]() Seven expression plasmids differing in the resistance gene, origin of replication, promoter sequence, and fusion protein tag were generated and transformed into four different E. coli expressing VvGT14ao, a glycosyltransferase gene from grape ( Vitis vinifera). The aim of this work was to increase the titre of terpenyl glucosides in biotransformation assays with E. However, there is still a need to optimize the biocatalysts. Glycosides are becoming increasingly more relevant for various industries as low-cost whole-cell-biocatalysts are now available for the manufacture of glycosides.
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